All primer sets worked under identical real-time PCR cycling conditions with similar efficiencies to obtain simultaneous amplification in the same run. Sequences were taken from GeneBank, all accessio
All primer sets worked under identical real-time PCR cycling conditions with similar efficiencies to obtain simultaneous amplification in the same run. Sequences were taken from GeneBank, all accessio
BRAF and MAP2K1 target regions are listed with the corresponding primer sequences and 5’ fluorophore tag. The underlined sequence represents the 17 nucleotides that allow size discrimination between t