Untargeted lipidomic profiling of the lung in G2019S LRRK2 knockin mice under a specialized diet

This Zenodo deposit contains a publicly available description of the Dataset: Title: "Untargeted lipidomic profiling of the lung in G2019S LRRK2 knockin mice under a specialized diet". Description: G2019S LRRK2 knockin mice and their wild-type littermates were maintained on a normal chow diet (23.7% kcal from fat) for the first 11 months of life. At that time, animals were randomly assigned to either a high-fat diet group (60% kcal from fat) or a control diet group (10% kcal from fat). Mice remained on their respective diets for an additional five months. At 16 months of age, mice were anesthetized with an isoflurane vaporizer, tissue were then collected and immediately snap frozen in liquid nitrogen. Frozen tissues were cryopulverized, homogenized and processed using the Bligh-Dyer method. Lipid identifications were assigned using LipidSearch (v5.0, Thermo Fisher Scientific) which was used to generate a compound list, and lipid peak areas were quantified in Skyline. Each experimental group contained biological replicates (n = 5-6 per group). This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset本Zenodo存档库包含该数据集的公开说明： 标题："特定膳食干预下G2019S突变型富亮氨酸重复激酶2（LRRK2）敲入小鼠肺部非靶向脂质组学分析" 研究说明：G2019S突变型富亮氨酸重复激酶2（LRRK2）敲入小鼠及其野生型同窝仔鼠在出生后的前11个月均饲喂普通维持饲料（脂肪供能占比23.7%）。待小鼠长至11月龄时，将其随机分为高脂饲料组（脂肪供能占比60%）与对照饲料组（脂肪供能占比10%），并持续饲喂对应饲料共计5个月。当小鼠至16月龄时，使用异氟烷麻醉机实施麻醉，随后采集肺组织并立即置于液氮中快速冷冻。将冷冻组织经低温粉碎、匀浆后，采用布莱-戴尔（Bligh-Dyer）提取法进行样本处理。脂质鉴定通过脂质搜索软件（LipidSearch，v5.0，赛默飞世尔科技（Thermo Fisher Scientific））完成，该软件用于生成化合物列表；脂质峰面积则通过Skyline软件进行定量。每个实验组均设置生物学重复（每组n=5~6）。 本数据集通过ASAP CRN云平台（cloud.parkinsonsroadmap.org）向研究人员开放，访问申请流程可参阅用户手册。 本研究由对齐帕金森病研究科学协作网络（Aligning Science Across Parkinson's Collaborative Research Network，简称ASAP CRN）通过迈克尔·J·福克斯帕金森病研究基金会（MJFF）资助。 本Zenodo存档库由ASAP CRN云平台工作人员代表数据集作者创建，可为该CRN云平台数据集提供可引用的文献标识。