Biological and physiological responses of Phyllospadix scouleri to simulated marine heatwaves

This dataset contains the raw and processed biological data supporting the study on the physiological responses of the surfgrass Phyllospadix scouleri under simulated marine heatwave conditions. The experiment evaluated the effects of three temperature treatments—control (18.5 ± 1.5°C), severe marine heatwave (23.5 ± 1.5°C), and extreme marine heatwave (26.5 ± 1.5°C)—across two experimental phases: warming and post-warming (recovery). Measurements were conducted on independent biological replicates (n = 4 per treatment × phase combination). The dataset includes ecophysiological descriptors spanning multiple functional domains: Photosynthesis and respiration: net and gross photosynthetic rates, respiration rate, photosynthesis–irradiance parameters (e.g., Pmax, Ek, α, Ec) Photophysiology: maximum and effective quantum yield (Fv/Fm, ΦPSII), electron transport rate (ETR), fluorescence parameters (F0, Fm), and non-photochemical quenching (NPQ) Nitrogen metabolism: nitrate uptake rates and nitrate reductase activity (NRA) Biochemical composition: chlorophyll a and b, carotenoids, phenolic content, antioxidant capacity Oxidative stress and damage: lipid peroxidation (MDA equivalents) Carbon balance and growth: non-structural carbohydrates (NSC), daily productivity (DP), and relative growth rate (RGR) Each observation is associated with metadata including treatment, experimental phase, and replicate identity. Data structure The dataset is provided as an Excel file containing: One sheet with biological response variables Columns representing: Treatment (Control, Severe-MHW, Extreme-MHW) Experimental phase (Warming, Post-warming) Measured variables Usage notes Values represent individual replicate measurements used in statistical analyses (ANOVA, PCA, PERMANOVA) reported in the associated manuscript. Units are consistent with those described in the manuscript methods and figure legends. Missing values correspond to measurements not available for specific variables or samples. Related publication This dataset supports the manuscript: Vivanco-Bercovich et al. (2026). Crossing thermal limits: functional collapse of the surfgrass Phyllospadix scouleri under simulated extreme marine heatwaves. Journal of Experimental Botany.本数据集包含支撑海浪草（Phyllospadix scouleri）在模拟海洋热浪（marine heatwave）条件下生理响应研究的原始与预处理生物学数据。 本实验设置三类温度处理：对照组（18.5 ± 1.5°C）、重度海洋热浪组（23.5 ± 1.5°C）与极端海洋热浪组（26.5 ± 1.5°C），并覆盖升温期与升温后（恢复）两个实验阶段。所有测量均采用独立生物学重复，每个处理×阶段组合的重复数为n=4。 本数据集涵盖多功能域的生态生理特征参数： 1. 光合与呼吸生理：净光合速率、总光合速率、呼吸速率、光合-光响应曲线参数（如最大光合速率Pmax、饱和光强Ek、初始量子效率α、光补偿点Ec等） 2. 光生理学：最大量子产率与有效量子产率（Fv/Fm、ΦPSII）、电子传递速率（ETR）、荧光参数（F0、Fm）及非光化学淬灭（NPQ） 3. 氮代谢：硝酸盐摄取速率与硝酸还原酶活性（NRA） 4. 生化组成：叶绿素a、叶绿素b、类胡萝卜素、酚类物质含量与抗氧化能力 5. 氧化应激与损伤：脂质过氧化水平（以丙二醛当量计） 6. 碳平衡与生长：非结构性碳水化合物（NSC）、日生产力（DP）及相对生长率（RGR） 每条观测数据均附带元数据，包括处理组信息、实验阶段与重复编号。 ### 数据结构 本数据集以Excel文件格式提供，包含一张存储生物学响应变量的工作表，其列信息如下： - 处理组（对照组、重度海洋热浪组、极端海洋热浪组） - 实验阶段（升温期、升温后恢复期） - 各测量变量 ### 使用说明 数据值为对应已发表论文中报道的统计分析（方差分析（ANOVA）、主成分分析（PCA）、置换多元方差分析（PERMANOVA））所用的个体重复测量数据。 单位与论文方法及图例中描述的标准保持一致。 缺失值代表特定变量或样本未获得有效测量的结果。 ### 相关出版物 本数据集支撑以下学术论文： Vivanco-Bercovich 等（2026）。《跨越热极限：模拟极端海洋热浪下海浪草（Phyllospadix scouleri）的功能崩溃》。《实验植物学杂志》（Journal of Experimental Botany）。