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Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera

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DataONE2023-12-18 更新2024-06-08 收录
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In this study, we detailed the indicators of development of H. armigera, such as growth and development period, number of surviving larvae, body weight, pupa weight, and number, as well as the relative expression levels of HaSumf1 gene at different instars, and the third- and fifth-instars after feeding with glucosinolates and /or dsSumf1., (1) H. armigera Rearing (2) Bioinformatics Analysis (3) Total RNA Extraction, cDNA Synthesis, and HaSumf1 Determination in Different Stages (4) Cloning and Sequencing HaSumf1 (5)  Synthesis of dsRNA in Vitro (6) Effects of Silencing HaSumf1 on the Growth and Development of H. armigera (7) Gene Expression after Silencing HaSumf1 (8) Statistical Analysis, , GENERAL INFORMATION 1. Title of Dataset: Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera [Access this dataset on Dryad](https://doi.org/10.5061/dryad.6q573n64w) 2. Author Information A. Principal Investigator Contact Information Name: Long Zhang Institution: Xinjiang University Email: B. Associate or Co-investigator Contact Information Name: Zhiran Zhou Institution: Xinjiang University Name: Fan Cai Institution: Xinjiang University Name: Wanli Qin Institution: Xinjiang University Name: Li Liu Institution: Xinjiang University Name: Guli Kuerban Institution: Xinjiang University Name: Lin Jin Institution: Nanjing Agricultural University Name: Xiaoli Ma Institution: Xinjiang University 3. Information about funding sources: National Natural Science Foundation of China, Award: 32060620; Natural Science Foundation of the Auto...

本研究详细记录了棉铃虫(Helicoverpa armigera,以下简称H. armigera)的生长发育相关指标,包括生长发育历期、幼虫存活数、虫体体重、蛹重及蛹的数量;同时检测了不同龄期幼虫,以及取食硫代葡萄糖苷和/或dsSumf1后的三龄、五龄幼虫体内HaSumf1基因的相对表达水平。本研究包含以下实验内容: (1) 棉铃虫饲养 (2) 生物信息学分析 (3) 总RNA提取、cDNA合成及不同发育阶段HaSumf1基因表达量检测 (4) HaSumf1基因的克隆与测序 (5) 体外合成dsRNA (6) 沉默HaSumf1基因对棉铃虫生长发育的影响 (7) 沉默HaSumf1基因后的基因表达分析 (8) 统计学分析 ## 数据集基本信息 1. 数据集标题:硫酸盐修饰因子1(HaSumf1)在棉铃虫生长发育及宿主硫代葡萄糖苷适应性中的功能 [可在Dryad数据库获取本数据集](https://doi.org/10.5061/dryad.6q573n64w) 2. 作者信息 A. 项目负责人联系方式 姓名:张龙 所属机构:新疆大学 电子邮箱: B. 副研究员或合作研究者联系方式 姓名:周智然,所属机构:新疆大学 姓名:蔡凡,所属机构:新疆大学 姓名:秦万里,所属机构:新疆大学 姓名:刘莉,所属机构:新疆大学 姓名:古丽·库尔班,所属机构:新疆大学 姓名:金琳,所属机构:南京农业大学 姓名:马晓丽,所属机构:新疆大学 3. 资助来源信息:中国国家自然科学基金(资助编号:32060620);新疆维吾尔自治区自然科学基金
创建时间:
2025-07-25
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