Figure S1 - Identification and Analysis of Putative Homologues of Mechanosensitive Channels in Pathogenic Protozoa

Identification of conserved residues in homologues of Piezo channel subunits. Multiple sequence alignment of predicted homologues of Piezo channel subunits from protozoa, vertebrates, Naegleria gruberi, D. discoideum, D. rerio, D. melanogaster, A. thaliana and O. sativa. The absolutely conserved PFEW motif and the surrounding five conserved predicted TMDs in human Piezo1 are highlighted with yellow shading. Open circles above the alignment indicate residues in human Piezo1 that are mutated in familial xerocytosis and alter channel gating [53]. An open triangle above the alignment indicates a position at which the introduction of a stop codon alters gating [53]. A filled circle above the alignment indicates the position of a single amino acid deletion in human Piezo2 that occurs in a subtype of Distal Arthrogryposis (E2727del) and alters channel gating [98]. Asterisks indicate absolutely conserved residues, while colons indicate residues with highly similar properties. Protozoan homologues with incomplete sequences (see Table 1) are not shown.
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