SMART-Seq analysis to understand the differences between WT and SRC-3-/- Tregs transcriptome

SMART-seq analysis is used for single cells or for a small amount of purified total RNA from low number of cells. The goal of this study to compare the quantitative differences between the SRC-3+ and SRC-3-/- Tregs cell transcriptomes. Method: Tregs were isolated from FOXP3cre SRC-3f/f (SRC-3KO) and FOXP3 cre monogenic (WT) mice and sorted for GFP expression and the expression of cre Transcriptome differences between WT and SRC-3KO Tregs explored to explain the anti-tumor effects of SRC-3KO Tregs