List of confirmed hit compounds.

Hit compounds of the primary screen were retested in replicates at 3 and 10 µM against HMGB1 and LPS and compounds are shown that decreased the HMGB-induced TNFα production by at least 40% in the hit confirmation experiments. (The majority of the glucocorticoids were not retested during the hit confirmation studies, since all glucocorticoids showed similar activity, confirming their class action.) TNFα production and viability values are shown for the primary screen and the TNFα production is shown for the hit confirmation experiments (Mean±SD). Compounds that reduced cell viability by at least 25% are labeled with an asterisk. (Abbreviations: Bay 11-7085: (2E)-3-[[4-(1,1-dimethylethyl)phenyl]sulfonyl]-2-propenenitrile, MNS: 3,4-methylenedioxy-β-nitrostyrene, PABA potassium salt: para-aminobenzoic acid potassium salt, NF-κB: nuclear factor κB, HIV: human immunodeficiency virus, Src: sarcoma tyrosine kinase, Syk: Spleen tyrosine kinase).