Chronic heat stress gene expression in mice liver

This study was done to know the transcritional changes occuring in mice liver in response to chronic heat stress at 34*c for a period of two weeks. Keywords: Chronic heat stress response on liver gene expression. The microarray study was done in Reference design. We used a in-house Rat-specific oligonucleotide microarray containing 1400 liver specific genes. Heat stress (HS) group had 6 mice, while thermoneutral group had 7 mice. Each microarray hybridization was done in using a reference design in which experimental sample was hybridized against a mouse reference RNA. For each biological sample, two or three technical replicates hybridizations were done out of which one hybridization was in swap dye design to detect dye specific bias.