Binding Changes in Chromatin Factors Upon Targeted XPO1 Inhibition in T Cells

We assessed the chromatin localization of XPO1 and p300 in response to C646 (10 uM), S109 (1 uM), and SP100030 (1 uM). We used CUT&RUN sequencing as a lower-input (500,000 cells per sample), higher-sensitivity alternative to ChIP-Seq to assess chromatin binding of different factors Overall design: Differential binding analysis and overlap analysis of XPO1 and p300 at basal conditions (DMSO), activated conditions (50 nM PMA + 1 uM Ionomycin), or activated + small molecule treatment in Jurkat T cells