Data for: Meloidogyne incognita (Nematoda: Meloidogynidae) sterol-binding protein Mi-sbp-1 as a target for its management

These files here are supporting data for the manuscript "Meloidogyne incognita (Nematoda: Meloidogynidae) sterol-binding protein Mi-sbp-1 as a target for its management". Supporting Information Legends Supporting Information 1: Supplementary figures 1 to 4. Supplementary figure 1- Amplification and copy number analysis of M. incognita Mi-sbp-1 gene Supplementary figure 2- The RNAi construct of Mi-sbp-1 for in-planta validation Supplementary figure 3- PCR confirmation of RNAi constructs of Mi-sbp-1 in tobacco transgenic events (T0) Supplementary figure 4- Detection of Mi-sbp-1 expression in T1 tobacco plants by qRT-PCR and effect of HIGS (host induced gene silencing) on transcript abundance of Mi-sbp-1 gene in feeding females. Supporting Information 2: A list of all the differentially expressed genes between dsRNA fed (FD) and starved (ST) nematode J2s as compared to control (FR) J2s, and information on genes of carbon and fat metabolism pathways as determined by the RNA-Seq experiment. The qRT PCR validation of expression of the selected carbon and fat metabolism pathway genes is also provided. Supporting information 3: A list of important pathways differentially expressed between dsRNA fed (FD) and starved (ST) nematode J2s as compared to control (FR) J2s, as identified by the GO-Elite analysis of the RNA-Seq experiment.

本批次文件为论文《"南方根结线虫（Meloidogyne incognita，线虫纲：根结线虫科（Meloidogynidae））甾醇结合蛋白Mi-sbp-1作为其防控靶标"》的配套支撑数据。
补充材料说明
补充材料1：补充图1至补充图4。
补充图1：南方根结线虫Mi-sbp-1基因的扩增与拷贝数分析
补充图2：用于植物体内验证的Mi-sbp-1 RNA干扰（RNA interference, RNAi）载体构建
补充图3：烟草T0代转基因植株中Mi-sbp-1 RNAi载体的PCR验证结果
补充图4：通过实时荧光定量PCR（quantitative real-time polymerase chain reaction, qRT-PCR）检测T1代烟草植株中Mi-sbp-1的表达水平，以及宿主诱导基因沉默（host induced gene silencing, HIGS）对取食雌虫体内Mi-sbp-1基因转录本丰度的影响。
补充材料2：基于RNA-Seq转录组测序实验，获取了喂食双链RNA（double-stranded RNA, dsRNA）的线虫二龄幼虫（FD组）、饥饿线虫二龄幼虫（ST组）相较于对照组（FR组）二龄幼虫的全部差异表达基因列表，以及碳代谢与脂肪代谢通路相关基因的相关信息；同时还提供了筛选出的碳、脂肪代谢通路基因的qRT-PCR表达验证结果。
补充材料3：通过对RNA-Seq转录组测序实验数据进行GO-Elite分析，获取了喂食双链RNA的线虫二龄幼虫（FD组）与饥饿线虫二龄幼虫（ST组）相较于对照组（FR组）二龄幼虫的显著差异表达通路列表。