BRG1-dependent regulation of Wnt signaling pathway during skeletal muscle differentiation

RNA-seq analysis for C2C12 myoblasts knocked down for either only-BRG1 or only-BRM or both BRG1 and BRM. Cells were treated with an siRNA SMARTpools of four oligos each targeting either BRG1 (siGENOME mouse Smarca4 pool #M-041135-01-0020) or BRM (siGENOME mouse Smarca2 pool #M-056591-00-0020) or both. Control samples were treated with non-targeting scrambled oligos (siRNA SMARTpool ON-TARGETplus scrambled non-targeting pool # D-001810-10-20). siRNA pools were purchased from Dharmacon, Horizon Discovery Ltd., USA. RNA-seq data was generated from undifferentiated and differentiated C2C12 cells. Cells were treated with siRNA oligos targeting either BRG1 or BRM or both BRG1 and BRM. Samples were harvested at proliferative stage (GM) and 48h post induction of differentiation (DM 48h). RNA from biological duplicates (for each treatment and timepoint) was extracted. Library preparation, sequencing and downstream analysis was performed by BGI Americas.