16S rRNA amplicon sequencing of the vaginal microbiota in first trimester miscarriage

To examine whether there are differences in the vaginal microbiota of women who miscarry compared to those who have normal pregnancy outcomes, we conducted a prospective observational study at the Canberra Hospital, Australia with 24 participant women in the first trimester of pregnancy. The V4 region of the 16S rRNA gene was amplified and sequenced using Illumina MiSeq 2x250 bp chemistry.