Summary of secondary reconstitutions in NSG mice.

2.5–5×105 expanded CD34+ CD133+ cells were transferred into the primary neonate or adult NSG recipients. 14–28 weeks after transfer, single cell suspension was prepared from the bone marrow of recipient mice. Human cells were isolated by either depleting mouse CD45+ cells or enriching for human CD34+ cells and then transferred into secondary neonate or adult recipients. Each secondary recipient received an equivalent of one femur's worth of enriched human cells. The numbers of CD34+ cells were calculated and shown to the nearest 10,000 cells. n.d. – not determined. 12 weeks after secondary transfer, chimerism was assessed in the bone marrow by staining for human and mouse CD45.