Predictive modeling of early childhood caries via spatial-temporal variations of oral microbiota (Validataion study). human oral metagenome

The strong correlation between bacterial communities and main factors (e.g., time and disease state) both between subjects and within subjects within a given niche thus suggested that oral microbiota could potentially model ECC onset. To test this hypothesis, the 34-children study cohort sampled two times during one year (T1, T3) were used as a train set for model construction, and an additional 24 child subjects were recruited and then each sampled at two time points (T3 and T4) for model validation: 14 patients including 7 experiencing caries-onset (HC group) and 7 experiencing caries- exacerbation (CC group) and ten healthy-steady controls (HH group).