Dataset related to the article "Modulation of soluble receptor for advanced glycation end-products (RAGE) isoforms and their ligands in healthy aging"

This record contains raw data related to the article "Modulation of soluble receptor for advanced glycation end-products (RAGE) isoforms and their ligands in healthy aging" The receptor for advanced glycation end-products (RAGE) recognizes several ligands involved in inflammatory diseases. Two circulating soluble isoforms exist: esRAGE derived from alternative splicing and cRAGE generated by the membrane-bound RAGE (FL-RAGE) proteolysis. Together, esRAGE and cRAGE constitute sRAGE and function as decoy receptors preventing FL-RAGE/ligands binding. We determined serum concentration of both, esRAGE and cRAGE, and their ligands AGEs, HMGB1 and S100A8/A9 in a healthy population of 169 subjects aged 20-90 years. cRAGE showed a negative (r=-0.375, P&lt;0.0001) while AGEs (r=0.160, P=0.0384) and S100A8/A9 (r=0.207, P=0.0091) a positive correlation with age. esRAGE did not change during aging and inversely correlated with Hemoglobin, ALT, insulin, HOMA index, Waist-Hip ratio (W/H), Waist Circumference (WC) and positively with AGEs. cRAGE exhibited also an inverse correlation with WC, W/H, PAI-1, HMGB1, AGEs and S100A8/A9. Age, W/H, HMGB1, S100A8/A9 and AGEs are independent predictors of cRAGE, whereas W/H and AGEs associate with esRAGE. Treatment of cells with glycated albumin reduced cRAGE production and upregulated FL-RAGE<em>. </em> These results indicate that in a healthy population cRAGE is a biomarker of aging while esRAGE represents a more reliable marker of obesity and insulin resistance. Hence, sRAGE isoforms levels could be differentially associated with age-related diseases risk factors.本数据集收录了与论文"Modulation of soluble receptor for advanced glycation end-products (RAGE) isoforms and their ligands in healthy aging"（《健康衰老过程中晚期糖基化终末产物受体异构体及其配体的调控》）相关的原始数据。晚期糖基化终末产物受体（receptor for advanced glycation end-products, RAGE）可识别多种参与炎症性疾病的配体。目前已发现两种循环可溶性异构体：由可变剪接产生的内泌型可溶性RAGE（endogenous secretory RAGE, esRAGE），以及由膜结合型RAGE（full-length RAGE, FL-RAGE）经蛋白水解生成的切割型可溶性RAGE（cleaved RAGE, cRAGE）。esRAGE与cRAGE共同构成可溶性RAGE（soluble RAGE, sRAGE），作为诱饵受体阻断FL-RAGE与配体的结合。本研究针对169名年龄介于20至90岁的健康人群，检测了其血清中esRAGE、cRAGE以及它们的配体晚期糖基化终末产物（advanced glycation end-products, AGEs）、高迁移率族蛋白B1（HMGB1）和S100钙结合蛋白A8/A9（S100A8/A9）的浓度。分析结果显示，cRAGE水平与年龄呈负相关（r=-0.375, P<0.0001），而AGEs（r=0.160, P=0.0384）与S100A8/A9（r=0.207, P=0.0091）水平则与年龄呈正相关。esRAGE水平未随衰老发生显著变化，但与血红蛋白、丙氨酸氨基转移酶（alanine aminotransferase, ALT）、胰岛素、稳态模型评估指数（Homeostatic Model Assessment, HOMA）、腰臀比（W/H）及腰围（WC）呈负相关，与AGEs呈正相关。cRAGE水平同样与WC、W/H、纤溶酶原激活物抑制剂-1（plasminogen activator inhibitor-1, PAI-1）、HMGB1、AGEs及S100A8/A9呈负相关。年龄、W/H、HMGB1、S100A8/A9及AGEs是cRAGE水平的独立预测因子，而W/H与AGEs则与esRAGE水平相关。用糖化白蛋白处理细胞可降低cRAGE的产生并上调FL-RAGE的表达。上述结果表明，在健康人群中，cRAGE可作为衰老的生物标志物，而esRAGE则是肥胖与胰岛素抵抗更为可靠的标志物。因此，可溶性RAGE异构体的水平可能与年龄相关疾病的风险因素存在差异化关联。