Raw Data on Extracellular Particles in 613 Human and 163 Canine Diluted Plasma and Blood Samples Assessed by Interferometric Light Microscopy

Extracellular nanoparticles (EPs) are cellular fragments. After being released in cell exterior, they become mediators of the cell-cell interaction. Their characterization in bodily fluids may reflect the clinical status of the organism. Here we present data on the number density n and hydrodynamic diameter Dh of EPs assessed directly in diluted plasma and blood by using a recently developed technique, Interferometric Light Microscopy (Romolo et al., 2022). The data are presented in the attached Table. We collected 613 blood and plasma samples from human patients with Inflammatory Bowel Disease (IBD) taken into tubes with trisodium citrate and ethylenediaminetetraacetic acid (EDTA) anticoagulants and 163 blood and plasma samples from canine patients with Brachycephalic Obstructive Airway Syndrome (BOAS). The human study was conducted in accordance with the Declaration of Helsinki, and approved by the National Medical Ethics Committee of the Republic of Slovenia (0120-271/2022/4; KME 27 July 2022). All procedures in the animal study complied with the relevant Slovenian government regulations (Animal Protection Act, Official Gazette of the Republic of Slovenia, No. 43/2007). The animal study was approved by the Animals in Experiments Welfare Commission of the Veterinary Faculty, University of Ljubljana, approval number 18-3/2022-1. Information regarding sample preparation is documented in the MIBlood-EV reports.细胞外纳米颗粒（Extracellular nanoparticles, EPs）即细胞片段。当其被释放至细胞外环境后，可作为细胞间相互作用的介导因子。对其在体液中的表征分析可反映机体的临床状态。本研究通过新近开发的干涉光显微镜技术（Interferometric Light Microscopy，Romolo等，2022），直接对稀释血浆及全血中的细胞外纳米颗粒进行检测，获取其数量密度n与流体力学直径Dh相关数据，相关结果已附于附表中。 我们共收集了613份来自炎症性肠病（Inflammatory Bowel Disease, IBD）患者的血液及血浆样本，采样时采用枸橼酸钠与乙二胺四乙酸（Ethylenediaminetetraacetic acid, EDTA）抗凝管；同时收集了163份来自短头阻塞性气道综合征（Brachycephalic Obstructive Airway Syndrome, BOAS）患犬的血液及血浆样本。 人体研究遵循《赫尔辛基宣言》开展，并经斯洛文尼亚共和国国家医学伦理委员会批准（审批号：0120-271/2022/4；KME，2022年7月27日）。动物研究的所有操作均符合斯洛文尼亚共和国相关政府法规（《动物保护法》，斯洛文尼亚共和国官方公报第43/2007号），并经卢布尔雅那大学兽医学院实验动物福利委员会批准，审批号为18-3/2022-1。样本制备相关信息详见MIBlood-EV报告。