Transcriptome analyses of in vitro exercise by EPS and adrenaline. Transcriptome analyses of in vitro exercise by EPS and adrenaline

To evaluate transcriptomic changes induced by in vitro exercise, we established two in vitro exercise models; EPS (electrical pulse stimulation and clenbuterol treatment). As for clen-buterol treatment, differentiated C2C12 myotubes were treated by 30 ng/ml clenbuterol for 1 hour and control and clenbuterol treated C2C12 myotubes were analyzed by RNA-sequencing. As for an EPS model, EPS was applied to differentiated C2C12 myotubes for 24 hours and control and EPS applied C2C12 myotubes were analyzed by RNA-sequencing. Overall design: Examination of 2 different in vitro exercise models (EPS and clenbuterol) in C2C12 myotubes.