Triticum aestivum strain:Jokwang, Jopum Transcriptome or Gene expression. Triticum aestivum strain:Jokwang, Jopum
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We performed RNA-Seq to detect differentially expressed wheat genes under high temperature conditions. More than 344 million short reads (100-bp paired ends) were generated using Illumina HiSeq technology. A comprehensive and integrated transcriptome was assembled from 83,939 transcripts by combining tentative consensus sequences using Trinity assembly software. Transcripts were annotated using Blast2GO, and differentially expressed transcripts were analyzed. A total of 208 upregulated and 182 downregulated transcripts were identified as involved in plastid, starch, and sucrose metabolism; glycerolipid metabolism; and glycerolipid and dicarboxylate metabolism. Our results demonstrate that RNA-Seq can be used successfully for gene identification and transcript profiling in wheat. Our sequence data provide a valuable resource for wheat researchers.



