Overexpression of AtCAMTA5 enhances drought tolerance in Arabidopsis.

The Overexpression of AtCAMTA5 leads to higher plant weight and seed weight as compare to Col-0. Under drought condition overexpression lines had reduce water loss,high water use efficiency decline in photosynthesis contributes to 95% survivability wherase Col-0 and camta5 showed enhance sensitivity.Microarray analysis revealed that AtCAMTA5 regulates genes enriched with CAMTA recognition motif like ANAC19, SPL16, aminotransferase, chitinase, MAPK7, SnRK2.2, E3-ligase, RUBISCO, PSI and PSII, etc which were involved in stress response, development, protein modification and photosynthesis. We used affymatrix expression analysis to validate role of CAMTA5 under drought stress along with physiological and biochemical assay. The total RNA of Col-0, camta5-a and Overexpresion (OX-5) lines were isolated under water and 14 days drought condition.The ATH1 gene chip was used for expression analysis.