Control or KZFP KD hESC-derived organoids RNA-seqs. Control or KZFP KD hESC-derived organoids RNA-seqs

Control or KD hESC were differentiated into organoids as decribed in Methods. RNA was extracted from individual organoids at day 20 after commencing the STEMdiff™ Cerebral Organoid Kit protocol. Day 0 was designated as the day of transfer of hESC to a 96-well plate for differentiation into embryoid bodies . Overall design: hESC were transduced with a control lentivector or a lentivector expressing a sh targeting the KZFPs, selected with puromycin and differentiated into cerebral organoids post-selection.