Differential expression profile of rat H9C2 cardiac cells following Hypoxia/reoxygenation (H/R)

Acute myocardial infarction is a leading cause of death among adults globally. Hypoxia/reoxygenation (H/R) injury plays a causal role in myocardial damage and death, whose molecular mechanisms remain largely elusive. Our present work employed rat H9C2 cardiomyocytes to establish an H/R injury model and investigated differential expression profiles following H/R treatment using RNA-seq. Normal cultured H9C2 cardiomyocytes were used as the control group. Overall design: H/R injury was established by culturing H9C2 cells in 1% oxygen, 5% CO2, and 94% nitrogen in a tri-gas incubator for 12 h and then reoxygenated in 5% CO2 normal oxygen conditions for 6 h. Thereafter, H9C2 cells (n=3) with or without H/R treatment were subjected to RNA isolation using the Trizol reagent (Life Technologies) and submitted to BGI for reverse transcription, library preparation, and sequencing using the BGI500 sequencer.