Replication Data for: triglycerides of "Replicative Senescence in Mesenchymal Stem Cells: An In Vitro Study on Mitochondrial Dynamics and Metabolic Alterations"

The Triglyceride Quantification Kit (MAK266-1KT, Sigma-Aldrich) was used to quantify TGs in bMSCs. The cells were seeded in a 6-well plate (Costar, Sigma-Aldrich) (1.5 × 105 cells/well). After 3 days, the cells were lysed in 1 mL of H2O + 5% NP-40 and centrifuged for 2 min at 13,000 rpm. Cells (6 × 103) were plated in triplicate in a 96-well black plate (Costar, Sigma-Aldrich). Lipase was then added to each well for 20 min at room temperature. The glycerol generated from the hydrolysis of TGs was then oxidized by adding the Master Reaction Mix provided in the kit, resulting in the generation of a fluorescent product (λex/λem = 535/587 nm). The fluorescence was acquired at Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific). A standard curve was generated to calculate the TG concentration in each sample (ng/μL).