TRPML1 Interactions Summary.

Qualitative assessment of interactions. Plus signs indicate interaction; minus signs indicate lack of interaction. Immunoprecipitation interaction strength was based on length of time before anti-V5 band appeared (anti-GFP bands appeared with 1 second of film exposure). Asterisk indicates that endogenous mouse STOML1 co-immunoprecipitates with GFP-TRPML1 in murine RAW264.7 macrophages. Split-Ubiquitin Yeast Two-Hybrid interaction strength was based on amount of growth on plates. Co-localization interaction strength is scored as one plus sign for every 25% co-localization (average). Dotted line separates candidate interactors identified by co-IP (above) or by Split-Ub YTH (below).