Molecular responses of TgWRKY24 transcription factor to abscisic acid signaling during seed dormancy in Tulipa thianschanica

China is rich in tulip resources. It is an important way to breed new tulipcultivars by distant hybridization using native wild Tulipa resources. T.thianschanica is an excellent germplasm resource distributed in China with brightyellow flowers and strong resistance. However, its seeds have deep dormancy.Transcriptome sequencing showed that a transcription factor, TgWRKY24, played animportant role in regulating seed dormancy in response to ABA treatment in tulip,but its molecular mechanism was unknown. This project intends to study themechanism of TgWRKY24 in ABA signal transduction and regulation of seed dormancy.The full length of TgWRKY24 will be cloned and the gene structure and tissuespecific expression will also be analyzed. The technologies of UPLC-MS/MS,qRT-PCR, Western bolting and VIGS will be used to investigate the expression ofTgWRKY24 in response to ABA during seed dormancy. Transcriptome sequencing resultswill be used to forecast the other genes in the ABA signaling pathway related tothe expression of TgWRKY24 and clone their promoters. The electrophoretic mobilityshift assay and a yeast one-hybrid system will be used to screen and verify thedownstream target genes of TgWRKY24. The results will be expected to explain thefunction of TgWRKY24 transcription factor in regulating tulip seed dormancy inresponse to ABA, and establish a new ABA signaling network for regulating seeddormancy in plant.