Immunofluorescence data of 700 host cell proteins in Sars-CoV2 infected vs non-infected VeroE6 cells

The data set contains ≈ 75,000 confocal images of mixed Vero E6 cell populations (SARS-CoV2 infected vs non-infected). Cells had been infected with the virus for 1 h and fixed 24 hour post infection in 96 well plates. Using immunofluorescence (4 colours) cells were stained for the SARS-CoV2 virus (red), the endoplasmic reticulum (yellow) and nucleus (blue) – same for all images in the data set and used as reference markers. On top of this, 700 different target proteins were stained – one per well (green). Images were acquired with an Opera Phoenix microscope at 63X, using 9 FoV and 3 z-planes per protein target. IN the original study, intensity differences and spatial re-distribution of the target proteins were evaluated in infected vs the non-infected cells. The image resource was generated and described in detail in this research article: (to be added).

本数据集包含约75000张混合Vero E6细胞群的共聚焦显微镜图像（confocal images），分为新型冠状病毒（SARS-CoV2）感染组与未感染组。细胞先以病毒感染1小时，于感染后24小时在96孔板中固定。本数据集采用4色免疫荧光染色（immunofluorescence）：所有图像均以针对新型冠状病毒（标记为红色）、内质网（endoplasmic reticulum，标记为黄色）与细胞核（nucleus，标记为蓝色）的染色作为通用参考标记；此外，700种不同的靶蛋白以绿色荧光标记，每孔对应一种靶蛋白。图像由Opera Phoenix显微镜（Opera Phoenix microscope）以63倍物镜采集，每个靶蛋白对应9个视野（Field of View，FoV）与3个Z轴层面（z-planes）。在原始研究中，研究者针对感染组与未感染细胞内靶蛋白的强度差异及空间重分布情况开展了评估。本图像数据集由该研究生成并详细阐释，相关研究论文信息待补充。