BM trephine - raw DSP count data

Determining the applicability of NanoString GeoMX to the analysis of BM trephine samples. 5 samples were analysed. MDS1, Normal, MDS2, AA1 and AA2. 12 x 300um ROIs were selected in each sample and analysed using GeoMX digital spatial profiling. For MDS1, Normal and MDS2 - ROIs 1-10 are in tissue, ROI 11 in decalcified trabeculum and ROI 12 in blank slide. For AA1 and AA2 - ROIs 1-11 are in tissue and ROI 12 is in blank slide. Raw counts and technical controls (pos and neg ERCC) are shown.

本研究旨在评估NanoString GeoMX（NanoString GeoMX）技术在骨髓（Bone Marrow, BM）环钻活检标本分析中的适用性。本研究共纳入5份样本完成分析，分别为MDS（Myelodysplastic Syndromes, MDS）1、正常对照（Normal）、MDS2、AA（Aplastic Anemia, AA）1及AA2。每份样本中选取12个300μm的感兴趣区域（Region of Interest, ROI），采用GeoMX数字空间分析技术进行检测。针对MDS1、正常对照及MDS2样本，ROI 1至10均位于组织区域，ROI 11位于脱钙小梁组织中，ROI 12位于空白玻片区域。针对AA1及AA2样本，ROI 1至11均位于组织区域，ROI 12位于空白玻片区域。本研究展示了原始计数数据及阳性、阴性ERCC（External RNA Controls Consortium, ERCC）技术对照结果。