Accepted species list of Eurotiales, including a DNA sequence reference database, as curated by the International Commission of Penicillium and Aspergillus (ICPA)

Eurotiales is a diverse and speciose order and includes economically important genera like Aspergillus, Penicillium, Paecilomyces and Talaromyces. Historically, species identifications based on morphology are challenging. The publication of accepted species lists and the availability of representative DNA sequences for type strains have contributed greatly towards accurate species identification and facilitated the description of many new species. However, despite current advancements, a proportion of newly described species within these taxonomically challenging genera represent, in fact, existing species, which raises obvious concerns. This study thus aimed to further modernise the taxonomy of Eurotiales by addressing key challenges in species identification and classification. Our study objectives were threefold: to review species described after 2023, update the accepted species list, and release a curated DNA sequence dataset to facilitate future species identifications. We conclude that a move to a phylogenetic species concept is necessary but continue to support the inclusion of morphological descriptions and, where possible, associated secondary metabolite, exoenzyme, physiology and ecological data when introducing new species. Our list now contains 1393 species classified into four families and 26 genera, with Aspergillus (n=465), Penicillium (n=598) and Talaromyces (n=236) containing the most species. To aid sequence-based identifications and species descriptions under a phylogenetic species concept, we release a curated DNA reference sequence database containing 18837 DNA sequences (3867 ITS, 5277 BenA, 5110 CaM and 4583 RPB2) generated from 5325 strains. Sequences were selected to best cover the infraspecies variation under our current understanding of each species. The sequence database will be kept up to date as new information becomes available. This manuscript presents a major leap towards our goal to facilitate work with Eurotiales, while providing the taxonomic framework to support research excellence related to this important fungal group. This dataset is curated and kept up to date by the International Commission of Penicillium and Aspergillus (ICPA). If you have questions or suggestions, please get in contact with ICPA members.Eurotiales（散囊菌目）是一个多样性丰富、物种繁多的目，包含具有重要经济价值的属，如Aspergillus（曲霉属）、Penicillium（青霉属）、Paecilomyces（拟青霉属）和Talaromyces（塔宾曲霉属）。长期以来，基于形态学的物种鉴定颇具挑战。经认可的物种名录的发布，以及模式菌株代表性DNA序列的可获取性，极大地推动了精准物种鉴定，并助力诸多新物种的描述。尽管当前已有研究进展，但这些分类学上颇具挑战的属内的部分新描述物种实际上属于已有物种，这一问题引发了显著的学界关切。 本研究旨在通过破解物种鉴定与分类中的关键难题，进一步推动散囊菌目分类学的现代化进程。本研究的目标分为三点：一是对2023年后发表的新描述物种进行梳理，二是更新经认可的物种名录，三是发布经过人工整理的DNA序列数据集，以助力后续的物种鉴定工作。我们认为，采用系统发育物种概念是必要之举，但同时也主张在发表新物种时，应纳入形态学描述，且在可行范围内附上相关的次级代谢产物、胞外酶、生理学及生态学数据。 本次更新的名录共收录1393个物种，隶属于4个科、26个属，其中Aspergillus（曲霉属，n=465）、Penicillium（青霉属，n=598）与Talaromyces（塔宾曲霉属，n=236）所含物种数量最多。为辅助基于序列的物种鉴定与系统发育物种概念下的新物种描述，我们发布了一套经过人工整理的DNA参考序列数据库，该库包含来自5325株菌株的18837条DNA序列（其中ITS序列3867条、BenA序列5277条、CaM序列5110条、RPB2序列4583条）。序列的选取充分覆盖了当前认知范围内每个物种的种内变异。该序列数据库将随着新研究数据的发布持续更新。本文为推动散囊菌目相关研究迈出了重要一步，同时也为这一重要真菌类群的相关高质量研究提供了分类学框架支撑。 本数据集由International Commission of Penicillium and Aspergillus（国际青霉与曲霉委员会，ICPA）进行整理维护并持续更新。如有疑问或建议，请联系ICPA成员。