Physiological data of Pacific oyster Crassostrea (Magallana) gigas after exposure to intermittent hypoxia and the combination with (fluctuating) elevated temperature

Organisms in intertidal zones experience fluctuations in environmental stressors such as hypoxia and temperature. These stressors and their fluctuations often appear in combination. Combination of stressors can have different effects compared to single stressors. In this study, we investigate the physiological effects of intermittent hypoxia in combination with different temperature regimes on the Pacific Oyster Crassostrea (Magallana) gigas. The oysters were exposed to hypoxic cycles (12h hypoxia by emersion/12h submersion) at normal (15°C), elevated (30°C) or fluctuating (15°C submersion/30°C emersion) temperature for 10 days. After the last submersion phase, the gills and digestive gland were sampled. We measured markers for bioenergetics and redox-balance in the gills and digestive gland using colorimetric methods as well as a set of metabolites (predominantly amino acids, osmolytes, anaerobic end products and energetic metabolites) in the gills using LC-MS/MS. Oysters kept submerged for up to 10 days were used as controls.潮间带生物会遭遇低氧、温度等环境胁迫因子的周期性波动。此类胁迫及其波动往往以复合形式出现，复合胁迫的生物学效应通常与单一胁迫存在差异。本研究以太平洋牡蛎（Crassostrea (Magallana) gigas）为实验材料，探究间歇性低氧配合不同温度制度对其产生的生理效应。实验将牡蛎分别置于常温（15℃）、高温（30℃）或波动温度（浸没阶段为15℃、暴露阶段为30℃）环境中，进行12小时出水低氧暴露与12小时水下浸没交替的低氧周期处理，持续10天。末次浸没阶段结束后，采集其鳃组织与消化腺样本。本研究通过比色法检测鳃组织与消化腺中生物能学及氧化还原平衡相关标志物，并采用液相色谱-串联质谱（LC-MS/MS）技术检测鳃组织中的一系列代谢物，主要涵盖氨基酸、渗透压调节物、厌氧终产物及能量代谢物。以持续浸没10天的牡蛎作为对照组。