Reproductive aging weakens offspring survival and constrains the telomerase response to herpesvirus in Pacific oysters

These datasets and codes report the impacts of aging on oyster parents and their offspring. We compared proteomic profiles (“parent_proteomic_rawdata.tsv”) of reproductive oysters (12 males and 12 females) of three age classes (young, middle age and old) (“parent_proteomic_design.txt”). We crossbred these adult oysters as described in the paper (DOI: 10.1126/sciadv.adq2311) to examine the impacts of parental aging on their offspring phenotypes. We explored the effects of reproductive aging on offspring growth (“offspring_growth_dataset.csv”) and survival rate at larval life stage from 0 to 40 days old(“larval_survival.csv”). We also measured spat resistance to OsHV1 in laboratory (“spat_mortality_OsHV1.csv”) and in the field (“spat_mortality_OsHV1_field.csv”), and offspring survival once they attained adulthood (“adult_survival.csv”). In parallel, we assayed intra- and inter-familial telomere length dynamics (“Telomere_dataset.csv”) and telomerase (“Telomerase_dataset.csv”) as described in the manuscript. We also uploaded the R codes used to analyse proteomic data on parents (“ADupoue - Analyses code-Parent proteomic.R”) and the impacts of parental aging on their offspring traits (“ADupoue - Analyses code-Offspring.R”). Finally, we provided the output of Markov Chain Monte Carlo models used to estimate narrow sense heritability in offspring traits (“growth_larvae.rds”, “growth_spat.rds”, “survival_larvae.rds”, “survival_OsHV1_lab.rds”, “survival_adult.rds”, “Telomere_preInf.rds”, “Telomere_postInf.rds”, “Telomerase_preInf.rds”, “Telomerase_postInf.rds”).本数据集与配套代码揭示了衰老对牡蛎亲本及其子代的影响。我们针对三个年龄组（幼年、中年、老年）的繁殖用牡蛎（12只雄性、12只雌性）的蛋白质组谱数据（"parent_proteomic_rawdata.tsv"）进行了比较分析，相关实验设计信息详见"parent_proteomic_design.txt"。我们按照论文所述方法（DOI: 10.1126/sciadv.adq2311）对这些成年牡蛎进行杂交，以探究亲本衰老对子代表型的影响。我们分析了生殖衰老对子代生长（"offspring_growth_dataset.csv"）以及0至40日龄幼虫阶段存活率（"larval_survival.csv"）的影响。此外，我们分别在实验室与野外环境下测定了幼贝对OsHV1的抗性，对应数据文件分别为"spat_mortality_OsHV1.csv"与"spat_mortality_OsHV1_field.csv"，同时记录了子代个体达到成年后的存活率（"adult_survival.csv"）。与此同时，我们按照手稿中的方法检测了家族内与家族间的端粒长度动态变化（"Telomere_dataset.csv"）以及端粒酶活性（"Telomerase_dataset.csv"）。我们还上传了用于分析亲本蛋白质组学数据的R代码（"ADupoue - Analyses code-Parent proteomic.R"），以及用于探究亲本衰老对子代性状影响的R代码（"ADupoue - Analyses code-Offspring.R"）。最后，我们提供了用于估算子代性状狭义遗传力的马尔可夫链蒙特卡洛（Markov Chain Monte Carlo）模型输出结果，对应文件包括"growth_larvae.rds"、"growth_spat.rds"、"survival_larvae.rds"、"survival_OsHV1_lab.rds"、"survival_adult.rds"、"Telomere_preInf.rds"、"Telomere_postInf.rds"、"Telomerase_preInf.rds"以及"Telomerase_postInf.rds"。