OMI experiment

<b>Cell metabolism in response to nutrient gradients</b>. A. Schematic representation of the experimental settings. HCT-116 cells were seeded at 30 million cells/mL in a collagen hydrogel. After 12h media was replaced by PBS following the described configuration. After 30h, OMI images were obtained. Then PBS was replaced with media again. Cells were cultured under media for another 20h (50 h post initial PBS change) before cells were imaged again. Cells were culture for 20 additional hours (70 and 40 h post initial PBS change and subsequent return to media). B-C OMI images (B) and quantification (C) of the ORR (Optical Redox Ratio) after 30 min in PBS/media, and at 20 and 40h after PBS was replaced by media. D-E OMI images (D) and quantification (E) of of NAD(P)H τ_m(mean lifetime) after 30 min in PBS/media, and at 20 and 40h after PBS was replaced by media. Significant main effects of different medias at the same time point are denotated by asterisks (*), significant comparisons between different time points are denotated by a hash (#) for the interface and a dagger (†) for PBS. For all symbols conferring statistical significance: single symbol p&lt;0.05, double symbol p&lt;0.01, triple symbol using One-Way ANOVA. For each condition a total of at least 40 cells from two independent experiments were analyzed with 3 Griddient plates per experiment.