CITE-seq profiling of human splenic dendritic cells

To depict a comprehensive atlas of human splenic DCs and investigate DC2hm in a broad context, we performed Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) using a panel of 151 oligo-conjugated antibodies recognizing surface proteins on Lin [CD3/CD19/NKp46]?CD66b? CD88?CD89?HLA-DR? splenocytes from two donors. This dataset identified nearly all currently recognized human splenic DC subsets. Integrated transcriptomic- and protein-level analyses support the conclusion that DC2hm represents a homeostatic maturation state originating from cDC2 and is distinct from other DC lineages. Overall design: Lin[CD3/CD19/CD56]?CD89?CD66b?CD88?HLA-DR? splenocytes from non-diseased adult human donors (trauma-induced splenectomy cases)