From Farm to Fork: Streptococcus suis as a Model for the Development of Novel Phage-Based Biocontrol Agents

These files are the supplementary data for the paper titled: From Farm to Fork: Streptococcus suis as a Model for the Development of Novel Phage-Based Biocontrol Agents(https://doi.org/10.3390/v14091996)AbstractBacterial infections of livestock threaten the sustainability of agriculture and public health through production losses and contamination of food products. While prophylactic and therapeutic application of antibiotics has been successful in managing such infections, the evolution and spread of antibiotic-resistant strains along the food chain and in the environment necessitates the development of alternative or adjunct preventive and/or therapeutic strategies. Additionally, the growing consumer preference for “greener” antibiotic-free food products has reinforced the need for novel and safer approaches to controlling bacterial infections. The use of bacteriophages (phages), which can target and kill bacteria, are increasingly considered as a suitable measure to reduce bacterial infections and contamination in the food industry. This review primarily elaborates on the recent veterinary applications of phages and discusses their merits and limitations. Furthermore, using Streptococcus suis as a model, we describe the prevalence of prophages and the anti-viral defence arsenal in the genome of the pathogen as a means to define the genetic building blocks that are available for the (synthetic) development of phage-based treatments. The data and approach described herein may provide a framework for the development of therapeutics against an array of bacterial pathogens.Keywords: phage; prophages; food; zoonosis; Streptococcus suis; anti-viral defence本数据集文件为下述论文的补充数据：《从农场到餐桌：以猪链球菌（Streptococcus suis）为模型开发新型基于噬菌体的生物防治制剂》（DOI: 10.3390/v14091996） 摘要：家畜细菌感染可通过造成生产损失及食品污染，威胁农业可持续发展与公共卫生安全。尽管抗生素的预防性与治疗性应用已成功管控此类感染，但耐药菌株沿食物链及环境演化并扩散，亟需开发替代或辅助性的预防与/或治疗策略。此外，消费者对无抗生素的“绿色”食品的偏好日益增强，进一步凸显了开发新型且更安全的细菌感染防控手段的必要性。能够靶向并杀灭细菌的噬菌体（bacteriophages，简称phages），正日益被视为降低食品工业中细菌感染与污染的可行方案。本综述主要阐述了噬菌体近年的兽医应用场景，并探讨了其优缺点与局限性。此外，本研究以猪链球菌（Streptococcus suis）为模型，详细描述了该病原菌基因组中的前噬菌体（prophages）分布情况与抗病毒防御系统，以此明确了可用于（合成）开发噬菌体治疗制剂的遗传元件。本文所述数据与方法可为针对一系列细菌病原体的治疗制剂开发提供参考框架。 关键词：噬菌体（phage）；前噬菌体（prophages）；食品；人畜共患病（zoonosis）；猪链球菌（Streptococcus suis）；抗病毒防御