Expression data from mesenchymal stem and progenitor cells freshly isolated from human bone marrow

Murine models of myeloid neoplasia show how leukemia infiltration alters the mesenchymal stem and progenitor cells to reinforce malignancy at the expense of healthy hematopoiesis. However, little is known about the bone marrow architecture in humans. We used global gene expression analyses to analyze mesenchymal stem and progenitor cells from AML patients and compared with mesenchymal stem and progenitor cells from the non-leukemic donor. Bone marrow mesenchymal stem and progenitor cells were isolated from either first diagnosed acute myeloid leukemia patients or non-leukemic donors with surface marker CD45-CD235a-CD31-CD271+CD146+