Downregulation of ADAM10 expression promotes neuronal differentiation in the developing spinal cord.

Two days after electroporation, the embryos were collected and transverse sections cut at E4 or E6. The transfected cells are marked by green fluorescence. The control morpholinos and the untransfected side of the spinal cord serve as controls. The immune reactive cells are stained red. Cell nuclei are labeled blue with 4′,6-diamidino-2-phenylindole (DAPI). (A–P) NeuN immunostaining (red) in sections transfected with control morpholinos (Fluo-m; A–D, I–L) and ADAM10 morpholinos (AM10-m; E–H, M–P) at E2 (A–H) or E4 (I–P), respectively. Arrows indicate the transfected region in the ventricular zone of the basal plate, arrowheads in (G) of the alar plate and arrowheads in (M–O) above the floor plate. Scale bar, 200 µm in (A) for (A–P).