Whole transcriptomic sequencing of CD133+CD44+ cancer stem cells from laryngeal squamous carcinoma cell line. Whole transcriptomic sequencing of CD133+CD44+ cancer stem cells from laryngeal squamous carcinoma cell line

To understand the gene expression profiling of cancer stem cells of laryngeal squamous carcinoma, the total RNA of CD133+CD44+ laryngeal cancer stem cells (isolated from LSCC cell line TU-177, named TDP), CD133-CD44- cells (TDN) and parental TU-177 (unsorted TU-177 cells, named TPT) was extracted, followed by RNA sequencing. Differentially expression of lncRNA, mRNA, and circRNA was identified. Overall design: The CD133+CD44+ cells (TDP) and CD133-CD44- cells (TDN) were isolated from TU-177 cells and cultured. Total RNA was extracted using TRizol reagent (Invitrogen, Carlsbad, CA) following the manufacturer’s instruction, followed by DNase I treatment to remove DNA contamination. Equal quantities of RNA from 3 samples in one group were mixed. The lncRNA library (including lncRNA, mRNA, and circRNA) was prepared. The library products were finally sequenced on Illumina HiSeq 2000 platform.