EdU Optimization - Media Tests #1
收藏DataCite Commons2022-07-20 更新2024-07-29 收录
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https://figshare.com/articles/dataset/EdU_Optimization_-_Media_Tests_1/20341023/1
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资源简介：
Flow cytometry data on mouse fecal bacteria incubated for three hours with (or without) 20 uM 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue in varying ratios of reduced Brain Heart Infusion (rBHI) broth and fecal slurry (FS), to capture the actively replicating bacteria. <br> The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria.
提供机构：
figshare
创建时间：
2022-07-20
