Ebolavirus diagnostic results of initial 29 specimens obtained from Bundibugyo District with numerical specimen numbers assigned.

RT-PCR refers to results obtained from conventional PCR using the broadly reactive Filo A/B primers [16]. Ag, IgM, and IgG refer to results from ELISA-based assays [7],[13] with Zaire ebolavirus reagents while virus isolation refers to culture attempts on Vero E6 cells [14]. Q-RT-PCR refers to results obtained using the optimized Bundibugyo ebolavirus specific real-time RT-PCR assay with cycle threshold (Ct) values of positive (Pos) samples indicated in the far right column.*Specimen # 200706291 is the clinical sample from which prototype isolate #811250 was obtained.