Multiomics Analyses Unveil Immune Landscape in Pediatric and Adult Sepsis [CITE-seq]

Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection. The immune response varies significantly based on the anatomical source of infection. This study uses cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) to profile surface protein expression and transcriptomics at the single-cell level in PBMC samples from 40 adults: 3 healthy controls, 5 ICU controls, and 32 sepsis patients. The sepsis patients are categorized by infections originating from different anatomical sites. The findings aim to support sepsis stratification and guide the development of targeted therapies based on infection sources. Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected from healthy adult controls, adult ICU controls, adult sepsis patients. CITE-seq was conducted to simultaneously measure surface protein expression and gene expression at the single-cell level. *************************************************************** The raw sequencing have been uploaded to China Genomic Sequence Archive with accession number GSA:HRA008452. ***************************************************************