PP1-NIPP1 wild type and mutant overexpression in Flip-In⢠T-Rex Hela cells
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RNAi-seq of stable Flp-In⢠T-Rex Hela cells that inducibly expressed PP1-NIPP1 and mutants. Overall design: Four cell types are investigated under two conditions in three independent experiments, resulting in 24 samples in total (i.e. 4x2x3). The four cell types are: (1) Flp-In⢠T-Rex Hela cells with doxycycline-inducible EGFP transgene, (2) Flp-In⢠T-Rex Hela cells with doxycycline-inducible EGFP-PP1-NIPP1 transgene, (3) Flp-In⢠T-Rex Hela cells with doxycycline-inducible EGFP-PP1m-NIPP1 transgene (PP1 is mutated at Aa 64, i.e. D64A), (4) Flp-In⢠T-Rex Hela cells with doxycycline-inducible EGFP-PP1-NIPP1m transgene (NIPP1 is mutated at the position AA 68-71, i.e. alanine mutation of NIPP1 residues 68-71). The two conditions are (1) not exposed to doxycycline, (2) treated with doxycycline. Three replicates are collected from three independent experiments.
