Table_5_Transcriptomic and Metabolomic Data Reveal the Key Metabolic Pathways Affecting Streltzoviella insularis (Staudinger) (Lepidoptera: Cossidae) Larvae During Overwintering.XLS

Streltzoviella insularis (Staudinger) (Lepidoptera: Cossidae) is a woodboring insect feeding on Fraxinus pennsylvanica, Sophora japonica, and Ginkgo biloba, as well as many other species used for urban greening and plain afforestation in northern China, including the temperate north. There is also a risk that S. insularis could spread through the transportation of seedlings, thereby increasing urban greening costs. However, how S. insularis increases the cold tolerance then reduces it to survive winter temperature below 0°C remains unclear. In the transcriptomic of S. insularis, we identified three profiles (profile 25, 27, and 13) whose trends related to the cold tolerance. We detected 1,783 differentially expressed genes (in profile 25) and identified 522 genes enriched in the AMPK signaling pathway. The metabolome analysis identified 122 differential metabolites. We identified four co-pathways, among which “Glycerophospholipid metabolism” was the pathway most enriched in differentially expressed genes and differential metabolites. The AMPK signaling and glycerophospholipid metabolism pathways play key roles in the natural overwintering physiological process of S. insularis larvae.

Streltzoviella insularis (Staudinger) (鳞翅目：合欢蛾科)是一种以Fraxinus pennsylvanica、Sophora japonica及Ginkgo biloba等众多物种为食的蛀木昆虫，这些物种在华北地区，包括温带北部，被广泛应用于城市绿化和平原造林。S. insularis存在通过种苗运输扩散的风险，从而增加城市绿化的成本。然而，S. insularis如何通过提高其耐寒性，然后在低于0°C的冬季温度下降低其耐寒性以生存，这一过程尚不明确。在S. insularis的转录组研究中，我们识别出三个与耐寒性趋势相关的轮廓（轮廓25、27和13）。在轮廓25中，我们检测到1,783个差异表达基因，并鉴定出在AMPK信号通路中富集的522个基因。代谢组分析确定了122个差异代谢物。我们鉴定出四种共途径，其中“甘油磷脂代谢”途径在差异表达基因和差异代谢物中最为富集。AMPK信号通路和甘油磷脂代谢途径在S. insularis幼虫自然越冬的生理过程中发挥着关键作用。