Supplementary Data for Bantan et al. (2026) "Foliar uptake of dissolved CO2: A distinct carbon acquisition pathway in crops"—pre-publication version

This repository contains the raw and processed stable-isotope data supporting Bantan et al., "Foliar uptake of dissolved CO₂: A distinct carbon acquisition pathway in crops." The study investigates whether terrestrial crop plants — lettuce (Lactuca sativa), tomato (Solanum lycopersicum), and cucumber (Cucumis sativus) — absorb and metabolically assimilate CO₂ from foliar-applied water. Plants were treated with ¹³C-labelled CO₂-infused water at varying concentrations (T25, T50, T75, T100), with surfactant-amended counterparts (ST25–ST100) and C0/C1 controls. δ¹³C was measured by EA-IRMS in whole shoot tissue (Experiments 1 and 2) and in extracted primary polar metabolites (Experiment 2). Files: Raw Isotope Data_Experiment 1_Bulk.xlsx — OEA Laboratories EA-IRMS report for Experiment 1 bulk shoot samples (per-sample δ¹³C VPDB, %C, calibration, QC). Raw Isotope Data_Experiment 2_Bulk.xlsx — OEA report for Experiment 2 bulk shoot samples. Raw Isotope Data_Experiment 2_Metabolites.xlsx — OEA report for Experiment 2 polar metabolite extracts. Master Data.xlsx — Two sheets (Bulk, Metabolites) compiling sample identifiers, treatments, fresh/dry weights, OEA-measured δ¹³C and %C, and derived quantities (atom fractions, ¹³C mass balance, excess ¹³C, uptake efficiency, Δδ¹³C) used to generate all figures and statistics in the manuscript.本仓库包含支撑Bantan等人发表于《叶面溶解态CO₂吸收：作物中独特的碳获取途径》（Foliar uptake of dissolved CO₂: A distinct carbon acquisition pathway in crops）的原始与经处理稳定同位素（stable-isotope）数据。本研究旨在探究陆生作物——生菜（Lactuca sativa）、番茄（Solanum lycopersicum）与黄瓜（Cucumis sativus）——是否能够吸收叶面施用水中的CO₂并完成代谢同化。实验采用不同浓度（T25、T50、T75、T100）的¹³C标记CO₂溶水溶液进行处理，同时设置添加表面活性剂的处理组（ST25至ST100）以及C0/C1对照组。研究通过元素分析仪-同位素比率质谱仪（EA-IRMS）测定了整株地上组织（实验1与实验2）以及实验2中提取得到的初级极性代谢物的δ¹³C值。 相关文件如下： 1. Raw Isotope Data_Experiment 1_Bulk.xlsx：OEA实验室针对实验1地上组织整体样品的EA-IRMS检测报告，包含单样品δ¹³C（VPDB校准）值、总碳百分比、校准结果及质量控制数据。 2. Raw Isotope Data_Experiment 2_Bulk.xlsx：OEA实验室针对实验2地上组织整体样品的检测报告。 3. Raw Isotope Data_Experiment 2_Metabolites.xlsx：OEA实验室针对实验2极性代谢物提取物的检测报告。 4. Master Data.xlsx：包含两个工作表（Bulk、Metabolites），汇总了样品标识符、处理组别、鲜/干重、OEA测定的δ¹³C值与总碳百分比，以及衍生计算量（原子分数、¹³C质量平衡、过剩¹³C、吸收效率、Δδ¹³C），上述数据用于生成论文中所有图表与统计分析结果。