Gut metabolites predict Clostridioides difficile recurrence

This repository contains: 1. Data used for analyses from: A) 16S rRNA gene amplicon analysis after paired-end Fastq files were truncated, filtered, denoised, and merged ["1-ASV_Counts.xlsx"] B) Untargeted metabolomics as provided by Metabolon ["2-UntargetedMetabolomics.xlsx"] C) Precision SCFA analysis ["3-PrecisionSCFA.xlsx"] D) Clincal demographic data of the study cohort ["Clinical Demographics.csv"] 2. Detailed results of univariate analyses in sub-folder "Univariate analysis results" for clinical data, untargeted metabolomics data, SCFAs, and ASVs 3. Detailed results of predictive analysis in subfolder "Predictive Analyses results", including per-fold performance results ("9-PredictiveResults.xlsx") and analysis of predictive features for ASVs ("10-ASVsPredictiveAnalysis.xlsx"), untargeted metabolites ("11-MetabsPredictiveAnalysis.xlsx"), targeted SCFAs ("12-SCFAPredictive Analysis.xlsx"), clinical data ("13-DemoPredictiveAnalysis.xlsx") and all data ("14-AllDataPredictiveAnalysis.xlsx")本数据集仓库包含如下内容：1. 支撑本研究分析的实验数据集，具体包括：A) 对双端Fastq文件完成截短、过滤、去噪及拼接操作后得到的16S rRNA基因扩增子测序分析数据，对应数据文件为"1-ASV_Counts.xlsx"，其中ASV即扩增子序列变异（Amplicon Sequence Variant）；B) 由Metabolon提供的非靶向代谢组学数据，对应文件为"2-UntargetedMetabolomics.xlsx"；C) 精准短链脂肪酸（SCFA, Short-Chain Fatty Acid）分析数据，对应文件为"3-PrecisionSCFA.xlsx"；D) 本研究队列的临床人口统计学数据，对应文件为"Clinical Demographics.csv"。2. 单变量分析详细结果，存放于子文件夹"Univariate analysis results"中，涵盖临床数据、非靶向代谢组学数据、短链脂肪酸及扩增子序列变异相关的全部分析结果。3. 预测分析详细结果，存放于子文件夹"Predictive Analyses results"中，具体包括：各折次模型性能结果文件"9-PredictiveResults.xlsx"，以及针对不同数据类型的预测特征分析结果：扩增子序列变异相关分析"10-ASVsPredictiveAnalysis.xlsx"、非靶向代谢物相关分析"11-MetabsPredictiveAnalysis.xlsx"、靶向短链脂肪酸相关分析"12-SCFAPredictive Analysis.xlsx"、临床人口统计学数据相关分析"13-DemoPredictiveAnalysis.xlsx"与全数据集相关分析"14-AllDataPredictiveAnalysis.xlsx"。