Reduced Representation Bisulfite Sequencing library (RRBS) preparation of SAM-treated A549
收藏DataCite Commons2026-04-13 更新2026-05-05 收录
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Genomic DNA (gDNA) was extracted from A549 cells following SAM treatment (8 hrs) using the Wizard Genomic DNA Purification kit (Promega). Purified gDNA (1 μg) was fragmented to an average size of 400 bp using a Covaris LE220R sonicator. Fragmented DNA was ligated to methylated adapters, followed by bisulfite treatment using the DNA Bisulfite Conversion Kit (Beyotime) according to the manufacturer’s protocol. The resulting single-stranded libraries were amplified using KAPA HiFi HotStart Uracil+ ReadyMix (Roche). Library concentration and size distribution were validated using a Qubit fluorometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer, respectively. RRBS was performed on an Illumina NovaSeq 4000 platform in 150 bp paired-end mode
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创建时间：
2026-04-13
