Linking Antigen Specific T cell Dynamics in a Microchip to Single Cell Transcription Patterns

A new screening profile has been realized that can aid in determining T-cell activation state at single-cell-level. Then, we performed single cell RNA sequencing to reveal the activation states of the cells from the dervied profiles can be indicative of the T-cell activation state. Comparative gene expression profiling analysis of scRNA-Seq data for OT-1 T cells that have interacted with OVA presenting antigen presenting cell and OT-1 cells that have interacted with nothing presenting antigen presenting cell.