Multiple Sclerosis Large Human Brain Tissue (MS Sample 2-White Matter Chronic Active Lesion) with 6 Spatial Transcriptomics Captures Using 10x Genomics Visium

This dataset was featured in the study: Scaling up spatial transcriptomics for large-sized tissues: uncovering cellular-level tissue architecture beyond conventional platforms with iSCALE. The dataset includes a large human brain tissue section from a patient with Multiple Sclerosis (MS) and contains a fully resolved chronic active white matter lesion, along with areas of active and chronic subpial cortical demyelination, surrounding immune response and normal-appearing white and gray matter. The sample spans an extensive tissue region and is accompanied by six 10x Genomics Visium Spatial Transcriptomics (ST) captures, each corresponding to smaller, partially overlapping subregions of the full tissue section, derived from adjacent slices. In addition, the data includes a high-resolution H&E-stained histology image of the entire large tissue section. Together, this dataset provides a rare opportunity to study both local and global spatial transcriptomic architecture of MS pathology across scales. Sample: MS Sample 2 Acknowledgement: Tissue samples and associated clinical and neuropathological data were supplied by the Multiple Sclerosis Society Tissue Bank, funded by the Multiple Sclerosis Society of Great Britain and Northern Ireland, registered charity 207495.本数据集源自以下研究：《面向大型生物组织的空间转录组学（Spatial Transcriptomics）规模化：依托iSCALE突破传统平台局限，揭示细胞级组织架构》。本数据集包含1例多发性硬化（Multiple Sclerosis, MS）患者的大块人体脑组织切片，涵盖一处完整解析的慢性活动性白质病变灶，同时包含活动性与慢性软膜下皮层脱髓鞘区域、周边免疫反应区域，以及外观正常的白质与灰质区域。 该样本覆盖了大范围组织区域，配套有6组10x Genomics Visium空间转录组测序（Spatial Transcriptomics, ST）捕获数据，每组数据对应完整组织切片中较小的、部分重叠的子区域，均取自相邻组织切片。此外，数据集还包含整块大型组织切片的高分辨率苏木精-伊红（hematoxylin-eosin, H&E）染色组织学图像。本数据集整体为跨尺度研究多发性硬化病理的局部与全局空间转录组架构提供了难得的研究契机。 样本：MS样本2 致谢：本研究的组织样本及相关临床、神经病理数据由多发性硬化协会组织库（Multiple Sclerosis Society Tissue Bank）提供，该库由英国及北爱尔兰多发性硬化协会资助，其注册慈善机构编号为207495。