Fertilization success rates from gamete age assays using eggs and sperm from Porites lobata corals in April-May 2023

This dataset includes fertilization success rates from gamete age assays using eggs and sperm from Porites lobata corals in April-May 2023. Porites lobata colonies were collected from study sites in the Rock Islands Southern Lagoon in the Republic of Palau. Colonies were isolated in individual plastic containers at the Palau International Coral Reef Center. Sperm from selected male colonies were pooled in a single container, and eggs were gently poured into this pool, allowing fertilization to commence. Fertilization success was evaluated visually 2-3 hours after fertilization began by observing a sub-sample of the eggs from each cross under a dissecting microscope and counting whole (undivided) eggs and dividing embryos.

本数据集包含2023年4至5月期间，以叶状微孔珊瑚（Porites lobata）的卵细胞与精子开展配子年龄试验所获得的受精成功率数据。试验所用叶状微孔珊瑚株采自帕劳共和国洛克群岛南部潟湖的研究样地，随后被安置于帕劳国际珊瑚礁中心（Palau International Coral Reef Center）的独立塑料容器中单独暂养。将选定的雄性珊瑚株所产生的精液汇集至同一容器内，随后轻柔将卵细胞倒入该混合精液体系中，启动受精过程。受精开始后2至3小时，通过体视显微镜（dissecting microscope）观察各杂交组合的卵亚样本，计数完整未分裂的卵细胞与分裂胚胎，以此完成受精成功率的目视评估。