Dopamine activity in the tail of the striatum, DeepLabCut and MoSeq during novel object exploration

In this study, we characterized dynamics of novelty exploration using multi-point tracking (DeepLabCut) and behavioral segmentation (MoSeq). Mice were habituated in an arena, and then a object was placed at the corner of the arena. We compared 4 groups of mice: one with presentation of a novel object (stimulus novelty), one with a presentation of a familiar object (contextual novelty), one with presentation of a novel object after ablation of dopamine neuorns that project to the tail of the striatum (TS), and one with presentation of a novel object after sham surgery. With a separate group of mice, dopamine activity in TS was recorded during novelty exploration., Novelty testing sessions consisted of animals exploring a single novel object within the behavioral arena. Object was placed in the corner of a behavioral arena (taped to floor to prevent animal from moving it, ~12-15cm from either wall). Sessions lasted for 25 minutes per animal per day for 4-12 days, and mice were run in the same order as habituation each day. One object was used per animal for duration of experiment and the objects were not shared between animals. Before each session, object would be submerged in soiled bedding (mixture of bedding from each mouse’s cage in current round, 6 animals) and wiped off with dry kimwipe to remove excess bedding dust. Objects were wiped with ethanol after each day and allowed to air out overnight before use.
For body part tracking, we used DeepLabCut version 1.0 (Mathis et al., 2018). Separate networks were used for different experimental settings: namely for mice without fiber implants (network A) and mice with fiber implants (network B). Bo..., , # Dopamine activity in the tail of the striatum, DeepLabCut and MoSeq during novel object exploration

There are data for 5 animal groups. Two groups of mice were presented with stimulus novelty or contextual novelty. Different two groups of mice were presented with stimulus novelty and dopamine neurons that project to the tail of the striatum (TS) were ablated or sham surgery was operated. A different group of mice were presented with stimulus novelty and dopamine activity in TS was recorded. All mice were recorded in videos and location of body parts was tracked with DeepLabCut. Some videos were also analyzed with Moseq.

## Description of the data and file structure

# Data structure

DLC (DeepLabCut) data is stored in .mat files. Given .mat files were named based on the following file structure:

 

.

+-- group1_name

|   +-- animal1_name

|       +-- session1_name

|           -- DLClabel.mat

|       +-- session2_name

|       +-- session3_name

|   +-- animal2_name

|   +-- an...

本研究采用多点追踪技术（DeepLabCut）与行为分割算法（MoSeq），对新奇探索行为的动态特征进行了表征。实验小鼠先在行为箱中适应环境，随后将一个物体放置于行为箱的角落处。
本研究共设置4组小鼠：一组呈现新奇物体（刺激新奇性组），一组呈现熟悉物体（情境新奇性组），一组在损毁投射至纹状体尾部（TS）的多巴胺能神经元后呈现新奇物体，还有一组在假手术后呈现新奇物体。此外，我们设置了另一组小鼠，在其进行新奇探索行为时记录其纹状体尾部的多巴胺活动。
新奇探索测试环节要求小鼠在行为箱内探索单个新奇物体：物体被粘贴于行为箱角落的地面（防止小鼠移动，距两侧墙壁约12~15cm）。每只小鼠每日的测试时长为25分钟，测试周期为4~12天，每日测试顺序与适应环境阶段一致。实验全程每只小鼠仅使用同一个物体，且不同小鼠间不共用测试物体。每次测试前，需将物体浸没于脏垫料中（垫料取自当前轮次6只小鼠各自笼盒的混合垫料），随后用无尘擦拭布擦去多余的垫料碎屑。每日测试结束后，需用乙醇擦拭物体，并置于通风处静置过夜以备后续使用。
在躯体部位追踪方面，本研究采用DeepLabCut 1.0版本（Mathis等，2018）。针对不同实验设置，我们分别使用了两套独立的神经网络：分别适配无光纤植入的小鼠（网络A）与有光纤植入的小鼠（网络B）。……# 纹状体尾部多巴胺活动、新奇物体探索过程中的DeepLabCut与MoSeq数据

本数据集共涵盖5组小鼠：2组分别接受刺激新奇性与情境新奇性处理；另外2组接受刺激新奇性处理，其中一组损毁了投射至纹状体尾部的多巴胺能神经元，另一组接受假手术；还有一组在接受刺激新奇性处理的同时记录其纹状体尾部的多巴胺活动。所有小鼠均录制了行为视频，其躯体部位坐标通过DeepLabCut进行追踪，部分视频还使用MoSeq进行了行为分析。

## 数据与文件结构说明
# 数据结构
DeepLabCut（DLC）数据以.mat格式文件存储。.mat文件的命名遵循以下文件结构：
.
├── 组1名称
│ ├── 动物1名称
│ │ ├── 测试环节1名称
│ │ │ └── DLClabel.mat
│ │ ├── 测试环节2名称
│ │ └── 测试环节3名称
│ ├── 动物2名称
│ └── 动物……