RNA-seq profiling of Methylotuvimicrobium alcaliphilum 20ZR grown with or without La-supplementation

In this study we investigated the steady-state growth of Methylotuvimicrobium alcaliphilum 20ZR in media containing calcium (Ca) or lanthanum (La, a REE element). RNA-seq profiling of Methylomicrobium alcaliphilum strain 20ZR in bioreactor on methane. Sample cultures, La-optimum, La-CH4 limited, Ca-optimum and Ca-CH4 limited, were collected and immediately transferred into tubes containing 5 ml of the stop solution (5% water-equilibrated phenol in ethanol). It was found, that cells supplemented with La show a higher growth rate compared to Ca-cultures; however, the efficiency of carbon conversion, estimated as biomass yield, is higher in cells grown with Ca. The study was financially supported by DOE under FOA DE-FOA-0001085 and by NSF-CBET award 1605031 Overall design: CaBR1: P3% CH4 +Ca +W +Cu CaBR2: P3% CH4 +Ca +W +Cu CaOLBR1: P3% low CH4 (methane limitation) +Ca +W +Cu CaOLBR2: P3% low CH4 (methane limitation) +Ca +W +Cu LaBR1: P(La)3% CH4 +La +W +Cu LaBR2: P(La)3% CH4 +La +W +Cu LaOLBR1: P(La)3% low CH4 (methane limitation) +La +W +Cu LaOLBR2: P(La)3% low CH4 (methane limitation) +La +W +Cu