Reproductive performance and sperm quality in the spermatheca of Apis mellifera queens as a function of fertilization method and floral availability

Data S1 - Raw data This file contains the complete raw dataset generated from the queen-rearing experiment and the subsequent evaluation of reproductive performance and spermathecal sperm quality in Apis mellifera queens. Data were obtained from queens reared in nucleus colonies and subjected to two fertilization methods (natural mating and instrumental insemination) under contrasting floral availability conditions (beginning of flowering and floral scarcity). The dataset includes individual level measurements of queen-rearing outcomes, oviposition performance, and spermathecal sperm parameters, including sperm concentration, motility, viability, acrosome integrity, mitochondrial activity, spermathecal volume, and egg-laying dynamics throughout the evaluation period. These raw data constitute the primary observations supporting the analyses and conclusions of the article entitled “Reproductive performance and sperm quality in the spermatheca of Apis mellifera queens as a function of fertilization method and floral availability”. Data S2 – Means ± Standard error This file contains the summarized dataset derived from the queen-rearing and reproductive performance experiment, expressed as mean values ± standard error (SE) for each parameter evaluated in Apis mellifera queens. Data are grouped according to fertilization method (natural mating and instrumental insemination) and floral availability (beginning of flowering and floral scarcity). The summarized values correspond to the descriptive statistics presented in the tables and figures of the associated article “Reproductive performance and sperm quality in the spermatheca of Apis mellifera queens as a function of fertilization method and floral availability” and facilitate comparative interpretation of treatment effects. Codebook´s This file contains the codebook for the datasets associated with the article “Reproductive performance and sperm quality in the spermatheca of Apis mellifera queens as a function of fertilization method and floral availability”. The codebook provides a detailed description of all variables included in both the raw and summarized datasets, including variable names, definitions, biological meaning, units of measurement, data types, and categorical coding schemes. It also specifies experimental factors related to queen rearing (Codebook S1), fertilization method (natural mating and instrumental insemination), floral availability (beginning of flowering and floral scarcity) (Codebook S2), and reproductive spermathecal stored sperm parameters (Codebook S3). Variable structure and naming conventions are consistent with the datasets and the statistical analyses performed in R software, facilitating reproducibility and correct interpretation of the data by other researchers.补充数据S1——原始数据 本文件包含源自西方蜜蜂（Apis mellifera）蜂王育王试验，以及后续对蜂王繁殖性能与储精囊精子质量评估所生成的完整原始数据集。数据采集自基于核群培育的蜂王，且在两种不同花源可利用条件（开花初期与花源匮乏期）下，接受了两种受精方式：自然交尾与人工授精。数据集包含育王结果、产卵性能以及储精囊精子参数的个体水平测量数据，具体包括精子浓度、活力、存活率、顶体完整性、线粒体活性、储精囊体积，以及整个评估周期内的产卵动态。这些原始数据构成了题为《蜜蜂蜂王受精方式与花源可利用性对其繁殖性能及储精囊精子质量的影响》的文章的分析与结论所依托的原始观测数据。 补充数据S2——均值±标准误（Standard Error, SE） 本文件包含源自育王与繁殖性能试验的汇总数据集，以西方蜜蜂蜂王各评估参数的均值±标准误（Standard Error, SE）形式呈现。数据按照受精方式（自然交尾与人工授精）与花源可利用条件（开花初期与花源匮乏期）进行分组。该汇总值对应关联文章《蜜蜂蜂王受精方式与花源可利用性对其繁殖性能及储精囊精子质量的影响》的图表中所呈现的描述性统计量，便于对处理组效应进行比较解读。 编码手册 本文件包含关联文章《蜜蜂蜂王受精方式与花源可利用性对其繁殖性能及储精囊精子质量的影响》所配套数据集的编码手册。该手册详细说明原始与汇总数据集中纳入的全部变量，包括变量名称、定义、生物学意义、测量单位、数据类型以及分类编码方案。同时明确了与育王相关的实验因素（编码手册S1）、受精方式（自然交尾与人工授精）、花源可利用条件（开花初期与花源匮乏期）（编码手册S2），以及储精囊储存精子的繁殖相关参数（编码手册S3）。变量结构与命名规范与数据集及R软件中执行的统计分析保持一致，便于其他研究人员复现实验并正确解读数据。