H3K27me3 modulates aberrant transcription and apoptosis in cyclophosphamide-induced ovarian granulosa cell injury [CUT&Tag]

Chemotherapy-induced ovarian damage and infertility are significant concerns for women of childbearing age with cancer, but the underlying mechanisms are still not fully understood. Our study revealed a close association between H3K27me3 and cyclophosphamide (CTX)-induced ovarian damage, especially in granulosa cells. To determine whether the up-regulation of selected genes induced by CTX treatment were associated with loss of H3K27me3 on specific gene regions, we performed an anti-H3K27me3 CUT&Tag experiment of GCs from the CTX-treated and the control groups using PBS (phosphate buffered saline) at 6 h after CTX or PBS treatment (or labeled as NT).